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close this bookBasic Laboratory Procedures in Clinical Bacteriology (WHO; 1991; 128 pages)
View the documentPreface
View the documentIntroduction
open this folder and view contentsQuality assurance in microbiology
close this folderPart I. Bacteriological investigations
open this folder and view contentsBlood
close this folderCerebrospinal fluid
View the documentIntroduction
View the documentCollection and transportation of specimens
View the documentMacroscopic inspection
View the documentMicroscopic examination
View the documentPreliminary identification
View the documentSusceptibility testing
open this folder and view contentsUrine
open this folder and view contentsStool
open this folder and view contentsLower respiratory tract infections
open this folder and view contentsUpper respiratory tract infections
open this folder and view contentsSexually transmitted diseases
open this folder and view contentsPurulent exudates, wounds, and abscesses
open this folder and view contentsAnaerobic bacteriology
open this folder and view contentsAntimicrobial susceptibility testing
open this folder and view contentsPart II. Essential media and reagents for isolation and identification of clinical pathogens
View the documentSelected further reading
View the documentSelected WHO publications of related interest
View the documentBack Cover
 

Collection and transportation of specimens

Approximately 5 - 10 ml of CSF should be collected in two sterile tubes by lumbar or ventricular puncture performed by a physician. In view of the danger of iatrogenic bacterial meningitis, thorough disinfection of the skin is mandatory. Part of the CSF specimen will be used for cytological and chemical examination, and the remainder for the microbiological examination. The specimen should be delivered to the laboratory at once, and processed immediately, since cells disintegrate rapidly. Any delay may produce a cell count that does not reflect the clinical situation of the patient.

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