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close this bookBasic Laboratory Procedures in Clinical Bacteriology (WHO; 1991; 128 pages)
View the documentPreface
View the documentIntroduction
open this folder and view contentsQuality assurance in microbiology
close this folderPart I. Bacteriological investigations
open this folder and view contentsBlood
open this folder and view contentsCerebrospinal fluid
close this folderUrine
View the documentIntroduction
View the documentSpecimen collection
View the documentCulture and interpretation
View the documentInterpretation of quantitative urine culture results
View the documentIdentification
View the documentSusceptibility tests
open this folder and view contentsStool
open this folder and view contentsLower respiratory tract infections
open this folder and view contentsUpper respiratory tract infections
open this folder and view contentsSexually transmitted diseases
open this folder and view contentsPurulent exudates, wounds, and abscesses
open this folder and view contentsAnaerobic bacteriology
open this folder and view contentsAntimicrobial susceptibility testing
open this folder and view contentsPart II. Essential media and reagents for isolation and identification of clinical pathogens
View the documentSelected further reading
View the documentSelected WHO publications of related interest
View the documentBack Cover
 

Identification

Identification should be performed as rapidly as possible. Since the vast majority of urinary tract infections are caused by E. coli, a rapid test should be used to identify red colonies from MacConkey agar.

β-Glucuronidase test for rapid identification of E. coli1

 

1 KILIAN, M. & BÜLOW, P. Rapid diagnosis of Enterobacteriaceae. I. Detection of bacterial glycosidases. Acta pathologica et microbiologica scandinavica. Section B, 84: 245-251 (1976).

1. Prepare a dense milky suspension of the organism to be tested in a small tube containing 0.25 ml of saline. The suspension should be prepared from colonies growing on MacConkey agar.

2. Add one tablet of 4-nitrophenyl-β-D-glucopyranosiduronic acid (PGUA) for detection of β-glucuronidase activity. Place a stopper in the tube and agitate vigorously for a few seconds.

3. Incubate the tube at 35-37 °C for 4 hours.

4. The development of a yellow colour in the supernatant indicates a positive test for E. coli.

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