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close this bookBasic Laboratory Procedures in Clinical Bacteriology (WHO; 1991; 128 pages)
View the documentPreface
View the documentIntroduction
open this folder and view contentsQuality assurance in microbiology
close this folderPart I. Bacteriological investigations
open this folder and view contentsBlood
open this folder and view contentsCerebrospinal fluid
open this folder and view contentsUrine
close this folderStool
View the documentIntroduction
View the documentCollection of faecal specimens
View the documentCollection of rectal swabs
View the documentExamination of specimens
View the documentPreparation of faecal suspension
View the documentInoculation of agar plates
open this folder and view contentsLower respiratory tract infections
open this folder and view contentsUpper respiratory tract infections
open this folder and view contentsSexually transmitted diseases
open this folder and view contentsPurulent exudates, wounds, and abscesses
open this folder and view contentsAnaerobic bacteriology
open this folder and view contentsAntimicrobial susceptibility testing
open this folder and view contentsPart II. Essential media and reagents for isolation and identification of clinical pathogens
View the documentSelected further reading
View the documentSelected WHO publications of related interest
View the documentBack Cover
 

Inoculation of agar plates

For the microbial culture of Shigella spp, Salmonella spp, E. coli and Yersinia enterocolitica, a general purpose plating medium of low selectivity (e.g., MacConkey agar) and another medium of moderate or high selectivity (e.g., deoxycholate citrate agar, or xylose-lysine-deoxycholate agar) should be used. For culture of Campylobacter spp, various specially designed selective media are available (e.g., Mueller-Hinton agar with 5 % sheep blood and others). Many Vibrio strains grow on MacConkey agar, although selective media, such as thiosulfate citrate bile salts (TCBS), sucrose agar, alkaline bile salt agar (BSA), and tellurite taurocholate gelatin (TTG), are preferable.

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