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close this bookBasic Laboratory Procedures in Clinical Bacteriology (WHO; 1991; 128 pages)
View the documentPreface
View the documentIntroduction
open this folder and view contentsQuality assurance in microbiology
open this folder and view contentsPart I. Bacteriological investigations
close this folderPart II. Essential media and reagents for isolation and identification of clinical pathogens
View the documentIntroduction
close this folderPriority grading of pathogens, culture media, and diagnostic reagents
View the documentBlood culture
View the documentCerebrospinal fluid specimens
View the documentUrine
View the documentStool culture
View the documentLower respiratory tract
View the documentUpper respiratory tract
View the documentUrogenital specimens for agents of sexually transmitted diseases
View the documentPus and exudates
View the documentList of recommended culture media and diagnostic reagents for the intermediate microbiological laboratory
View the documentSelected further reading
View the documentSelected WHO publications of related interest
View the documentBack Cover
 

Stool culture

Common pathogens

Pathogens with high priority

 

Salmonella typhi and S. paratyphi
Shigella
Vibrio cholerae serogroup 01, noncholerae vibrios, Aeromonas and Plesiomonas

Pathogens with intermediate priority

 

non-typhoid salmonellae and Edwardsiella
Yersinia enterocolitica
Campylobacter jejuni
Clostridium difficile

Pathogens with low priority

 

Escherichia coli (enteropathogenic, enterotoxigenic, enteroinvasive, and enterohaemorrhagic)

Culture media and diagnostic reagents

Transport media

Priority code

Cary-Blair medium (for all pathogens)

1

buffered glycerol saline (not for Vibrio and Campylobacter)

2

Enrichment media

 

selenite F broth

2

alkaline peptone water

2

Isolation media

 

MacConkey agar (with crystal violet)

1

deoxycholate citrate agar (can be replaced by Salmonella-Shigella agar)

1

TCBS agar (can be replaced by Monsur’s TTG agar)

1

Campylobacter medium: Columbia agar base or any blood agar base with lysed blood and antibiotic supplement

2

Clostridium difficile selective medium

3

Preliminary identification media and reagents

 

Kligler iron agar (KIA) (may be replaced by triple sugar iron agar (TSI) but only for enteric pathogens)

1

motility-indole-urease medium (MIU) (may be replaced by motility test medium + peptone urea broth)

1

Kovac’s reagent for indole

1

oxidase reagent

1

Specific identification media and reagents

 

Biochemical

 

ONPG test (disc)

2

lysine decarboxylase broth (Möller base)

2

Simmons’ citrate agar

2

Andrade peptone water (or phenol red broth base)

2

acetate agar

3

DNase agar

3

vibriostatic compound 0/129 disc

3

Serological: agglutinating antisera

 

Priority code

Salmonella:

polyvalent 0

1

S. typhi:

anti OD (9, 12), anti H: d, anti Vi

1

S. typhimurium:

anti 0: 4, 5 (B), anti H: i

2

Shigella:

polyvalent flexneri, sonnei, boydii, dysenteriae, specific anti-Shigella dysenteriae 1 (Shiga)

1

Vibrio:

polyvalent 0:1

1

 

specific anti-ogawa and anti-inaba

3

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